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Development and characterization of 20 new polymorphic microsatellite markers from Mangifera indica (Anacardiaceae)
Authors:Chiang Yu-Chung  Tsai Chi-Mou  Chen Yu-Kuang H  Lee Sheue-Ru  Chen Chih-Hsiung  Lin Yu-Shium  Tsai Chi-Chu
Institution:Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Abstract:? Premise of the study: Twenty microsatellite loci for mango (Mangifera indica), an important commercial fruit tree in East Asia, were developed to evaluate the genetic diversity and identification of cultivars. ? Methods and Results: The 20 new microsatellite markers were isolated from mango using a magnetic bead enrichment method, and polymorphisms were identified in 22 mango cultivars. The number of alleles ranged from one to nine, with expected heterozygosity ranging from 0 to 0.826. The polymorphism information content ranged from 0 to 0.756 with a mean of 0.525. ? Conclusions: These new microsatellite loci should be useful and convenient for further studies of the genetic diversity and identification of cultivars in mango.
Keywords:Anacardiaceae  cultivar identification  genetic diversity  Mangifera indica  microsatellite DNA
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