Development and characterization of 20 new polymorphic microsatellite markers from Mangifera indica (Anacardiaceae) |
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Authors: | Chiang Yu-Chung Tsai Chi-Mou Chen Yu-Kuang H Lee Sheue-Ru Chen Chih-Hsiung Lin Yu-Shium Tsai Chi-Chu |
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Institution: | Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan. |
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Abstract: | ? Premise of the study: Twenty microsatellite loci for mango (Mangifera indica), an important commercial fruit tree in East Asia, were developed to evaluate the genetic diversity and identification of cultivars. ? Methods and Results: The 20 new microsatellite markers were isolated from mango using a magnetic bead enrichment method, and polymorphisms were identified in 22 mango cultivars. The number of alleles ranged from one to nine, with expected heterozygosity ranging from 0 to 0.826. The polymorphism information content ranged from 0 to 0.756 with a mean of 0.525. ? Conclusions: These new microsatellite loci should be useful and convenient for further studies of the genetic diversity and identification of cultivars in mango. |
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Keywords: | Anacardiaceae cultivar identification genetic diversity Mangifera indica microsatellite DNA |
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