Use of RAPD markers to determine the genetic diversity of diploid,wheat genotypes |
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Authors: | Richard A Vierling Henry T Nguyen |
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Institution: | (1) Plant Genetics Laboratory, Department of Agronomy, Horticulture and Entomology, Texas Tech University, 79409 Lubbock, Texas, USA;(2) Present address: Genetics Laboratory, Indiana Crop Improvement, 3510 US 52 South, 47905 Lafayette, Indiana, USA |
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Abstract: | Summary The genetic diversity of two diploid wheat species, Triticum monococcum and Triticum urartu (2n=2x=14), was assessed using random primers and the polymerase chain reaction (PCR). Electrophoretic analysis of the amplification products revealed a higher incidence of polymorphism in T. urartu than T. monococcum. Pair-wise comparisons of unique and shared polymorphic amplification products, were used to generate Jaccard's similarity coefficients. These were employed to construct phenograms using an unweighted pair-group method with arithmetical averages (UPGMA). The UPGMA analysis indicated a higher similarity among T. monococcum than T. urartu. Analysis of RAPD data appears to be helpful in determining the genetic relationships among genotypes.Contribution of the College of Agricultural Sciences, Texas Tech University Journal No. T-4-334. This work was supported by a grant from the National Science Foundation (BSR-8552915) |
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Keywords: | RAPD Genetic diversity Phenograms Triticum monococcum Triticum urartu |
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