Zinc compounds inhibit osteoclast-like cell formation at the earlier stage of rat marrow culture but not osteoclast function |
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Authors: | Masayoshi Yamaguchi Seiko Kishi |
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Institution: | (1) Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City 422, Japan;(2) Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, Shizuoka City 422, Japan |
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Abstract: | The effect of zinc compounds on osteoclast-like cell formation in rat marrow culture in vitro was investigated. The bone marrow cells were cultured for 7 days in -minimal essential medium containing a well-known bone resorbing hormone (1, 25-dihydroxyvitamin D3 and parathyroid hormone 1–34]). Osteoclast-like cell formation was estimated by staining for tartrateresistant acid phosphatase (TRACP), a marker enzyme of osteoclasts. The presence of 1, 25-dihydroxyvitamin D3 (10–8 M) or parathyroid hormone (PTH; 10–8 M) induced a remarkable increase in osteoclast-like multinucleated cells (MNC). These increases were clearly inhibited by the presence of zinc sulfate or zinc-chelating dipeptide ( -alanyl-L-histidinato zinc; AHZ) in the concentration range of 10–7 to 10–5 M. The inhibitory effect was seen at the earlier stage of osteoclast-like MNC formation. However, zinc compounds (10–6 M) did not have an effect on PTH (10–8 M)-induced osteoclast-like cell formation in the presence of EGTA (5 × 10–4 M), dibucaine (10–5 M) or staurosporine (10–9 M). Moreover, when osteoclasts isolated from rat femoraldiaphyseal tissues were cultured for 24 h in the presence of zinc compounds (10–7 to 10–5 M), the compounds did not have an effect on cell numbers or lysosomal enzymes activity (acid phosphatase and -glucuronidase) in the cells. The present study clearly demonstrates that zinc compounds inhibit osteoclast-like cell formation at the earlier stage with differentiation of marrow cells. |
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Keywords: | zinc -alanyl-L-histidinato zinc" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-alanyl-L-histidinato zinc osteoclast formation bone marrow cells bone metabolism |
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