ENZYMIC FORMATION OF GLUTAMINE IN RICE-PLANT SEEDLINGS |
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Authors: | IZUMI KUNIHIKO |
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Institution: | Department of Chemistry, Faculty of Science, Kyoto University Kyoto |
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Abstract: | - Purified preparation from rice-plant seedling catalyses a stoichiometricreaction between ATP, glutamate, and NH2OH in the presence ofMg++ to form glutamyl hydroxamate, ADP and inorganic phosphate.
- The method of purification and some of the properties of theenzyme are described. Co++ can be substituted for Mg++. Mn++,NaF, and PCMB inhibit the enzyme strongly.
- Inorganic orthophosphateis liberated from ATP by the additionof
or cysteine in the presence of glutamate, Mg++ andthis preparation. Glutamine was detectedin the reaction productsby paperchromatography. - The same preparation catalyses a reactionbetween gluta mineand NH2OH in the presence of Mg++ or Mn++,ADP and inorganicphosphate, to form glutamyl hydroxamate.
1 Present address: The Department of Chemistry, Faculty of Science,Kanazawa University, Kanazawa. (Received October 31, 1960; ) |
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