转基因构件在哺乳动物外源基因整合、表达与调控中的作用

哺乳动物的转基因研究自八十年代初发展起来后已迅速、广泛应用于研究真核生物基因表达调控机制、建立人类疾病动物模型、动物育种、基因产品的制备等方面，已成为分子生物学技术中不可缺少的内容。随着转基因技术的发展，外源基因整合的随机性和表达的不可调节性正逐步得以克服。从随机整合到定位整合，从基因加入到基因剔除和基因精确修饰，从表达的不可调节到可控表达，转基因研究的应用前景正朝着无限可能的方向发展。但目前的技术还面临着某些不足，如效率较低、刺激诱导引起的多向效应、特异性不高、有较强的背景活性。本文将从外源基因的角度探讨不同构件对基因整合、表达和调控的影响，以期为外源基因的构建提供有益的尝试。

The Functions of Transgenic Components on Integration,Expression and Control of Exogenous Gene

The application of transgenic study on mechanism of gene expression and control, establishment of human disease model, animal breeding and preparation of transgenic production ect, has become more and more in depth. From random integration to site-specific recombinaton, from gene insertion to knoch out, from irregulary to controlable expression, transgene technology is rapidly developing to infinit potentialiies. In this paper, we will discass the effect of different transgenic components on the integration, expresion and control of exogenous gene.