Lack of ceruloplasmin expression alters aspects of copper transport to the fetus and newborn, as determined in mice |
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Authors: | Yu-Ling Chu Eric N Sauble Anthony Cabrera Aleeza Roth M Leigh Ackland Julian F B Mercer Maria C Linder |
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Institution: | (1) Department of Chemistry and Biochemistry, California State University, Fullerton, CA 92834-6866, USA;(2) Centre for Cellular and Molecular Biology, Deakin University, Burwood, VIC, 3052, Australia; |
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Abstract: | Copper transport and accumulation were studied in virgin and lactating C57BL/6 mice, with and without expression of ceruloplasmin
(Cp), to assess the importance of Cp to these processes. One hour after i.p. injection of tracer 64Cu, liver and kidney accounted for 80% of the radioactivity, and mammary gland 1%, while in lactating Cp+/+ mice 2–4 days
post partum, uptake by mammary gland was 9-fold higher and that of liver and other organs was decreased, with 64Cu rapidly appearing in milk. Parallel studies in Cp−/− mice (siblings from same colony) gave virtually identical results.
However, their milk contained less 64Cu, and actual copper contents determined by furnace atomic absorption were less than half those for milk from normal dams.
Liver copper concentrations of pups born to Cp−/− dams also were half those of pups from wild type dams. Copper in pup brains
was unaffected; but iron concentrations were reduced. We conclude that absence of Cp, while not affecting entry of exchangeable
copper from the blood into the mammary gland, does have a significant effect on the availability of this metal to the newborn
through the milk and in the form of stores accumulating in gestation. |
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