Similar developmental patterns in immunolocalisation of stem cell factor and KIT in bovine meso- and metanephros |
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Authors: | Nikoloz Tsikolia Michiharu Sakurai Katharina Spanel-Borowski Albert M Ricken |
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Institution: | 1. Institute of Anatomy, Medical Faculty, University of Leipzig, Liebigstra?e 13, 04103, Leipzig, Germany 2. Molecular Biology and Immunology Research Division, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-0856, Japan
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Abstract: | The mesonephros is often regarded as a simplified version of the terminal renal organ, the metanephros. Both renal organs
result from an epithelio-mesenchymal interaction between the Wolffian duct and the nephrogenic ridge. It appears that the
epithelio-mesenchymal interaction makes use of similar signal cascades for both renal organs and that key events required
for the development of the metanephros occur at earlier stages. In murine metanephroi, the stem cell factor (SCF)/-KIT-signal transduction pathway has recently been shown to regulate ureteric
bud branching and epithelial cell differentiation. We immunohistochemically defined the time-sequence of KIT and SCF presence
in both renal organs using bovine embryos/foetuses with crown rump length (CRL) of 1.7–24 cm. In the mesonephroi, epithelial
cells with strong KIT staining were scattered in distal tubules, and SCF was expressed in the epithelial wall of corpuscles
and proximal tubules. KIT positivity occurred in the metanephroi of embryos prior to SCF; KIT was predominantly localised
at the ureteric bud tips in the nephrogenic zone. In foetuses of 13 cm and more CRL, the SCF/KIT profile of developmentally
advanced nephrons mirrored the situation in the mesonephros. Epithelial cells with strong KIT staining were scattered in the
cortical areas of distal tubules, while SCF was expressed in the epithelial wall of corpuscles and proximal tubules. Our morphological
findings agree with a potential role of KIT at the ureteric bud tips and demonstrate a similar expression of KIT and SCF along
the areas of developmentally advanced mesonephric and metanephric nephrons. |
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