Identification of <Emphasis Type="Italic">Chlamydomonas reinhardtii</Emphasis> Rad51C: Recombinational characteristics |
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| Authors: | V I Shalguev O K Kaboev I A Sizova P Hagemann V A Lanzov |
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| Institution: | (1) Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, 188300 Gatchina, Leningrad Region, Russia;(2) St. Petersburg State University, 198904 St. Petersburg, Russia;(3) Institute für Biochemie, Genetik, and Microbiologie, Universität Regensburg, 93040, Germany |
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| Abstract: | Unicellular green alga Chlamydomonas reinhardtii is a promising model for fundamental and biotechnological research. However, little is known about its system of homologous recombination underlying recombination repair of double-strand breaks. Sequencing of the C. reinhardtii nuclear genome has revealed many repeats, which account for a low level of nuclear homologous recombination compared to that of nonhomologous recombination. Analysis of C. reinhardtii EST and genomic libraries made it possible to reconstruct and clone the RAD51C cDNA. In this work, this cDNA was expressed, the protein product was purified, and its main biochemical activities were studied. It was shown that Rad51C of lower eukaryote C. reinhardtii is a typical member of the subfamily of higher eukaryotic Rad51-like recombination proteins.Translated from Molekulyarnaya Biologiya, Vol. 39, No. 1, 2005, pp. 112–119.Original Russian Text Copyright © 2005 by Shalguev, Kaboev, Sizova, Hagemann, Lanzov. |
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| Keywords: | Chlamydomonas reinhardtii Rad51C homologous recombination biochemical activities of DNA transferases |
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