| 登革病毒转基因载体pB［PUBnls_EGFP_prM］的构建及其在C6/36细胞中整合作用的检测 |
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| 引用本文： | 葛春喜,黄炯烈,陈观今,吴瑜,于洪枫. 登革病毒转基因载体pB［PUBnls_EGFP_prM］的构建及其在C6/36细胞中整合作用的检测[J]. 昆虫学报, 2003, 46(5): 549-552 |
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| 作者姓名： | 葛春喜 黄炯烈 陈观今 吴瑜 于洪枫 |
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| 作者单位： | 中山大学中山医学院病原生物学教研室,广州,510080 |
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| 基金项目： | “2 11工程”重点学科建设基金资助项目 ( 9812 4) |
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| 摘 要： | 利用转基因技术来探索新的蚊媒疾病防治方法，将登革病毒前膜蛋白基因prM重组入以转座子piggyBac因子为基础的载体，构建了昆虫转基因载体pB［PUBnls-EGFP-prM］，在辅助质粒的作用下共同转染白纹伊蚊Aedes albopictus C6/36细胞。PCR和Southern blot证明构建的转基因载体可以将EGFP-prM基因整合入蚊虫基因组中。验证了转座子piggyBac因子、启动子polyubiquitin可以在白纹伊蚊中发挥功能,为进一步构建不传播登革病毒的转基因白纹伊蚊奠定了基础。
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| 关 键 词： | 白纹伊蚊C6/36细胞 prM基因 piggyBac因子 转座子 |
| 文章编号： | 0454-6296(2003)05-0549-04 |
| 修稿时间： | 2002-07-24 |
Construction of transgenic vector pB [PUBnls-EGFP-prM] and identification of its integrational function in mosquito cell |
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| GE Chun-Xi,HUANG Jiong-Lie,CHEN Guan-Jin,WU Yu ,YU Hong-Feng. Construction of transgenic vector pB [PUBnls-EGFP-prM] and identification of its integrational function in mosquito cell[J]. Acta Entomologica Sinica, 2003, 46(5): 549-552 |
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| Authors: | GE Chun-Xi HUANG Jiong-Lie CHEN Guan-Jin WU Yu YU Hong-Feng |
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| Affiliation: | GE Chun-Xi,HUANG Jiong-Lie,CHEN Guan-Jin,WU Yu *,YU Hong-Feng |
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| Abstract: | To explore a new method to control arthropod-borne diseases, prM gene from dengue virus was inserted into a transposon piggyBac based plasmid and an insect transgenic vector pB[PUBnls-EGFP-prM] was constructed. The vector was used to transfect Aedes albopictus C6/36 cell with a helper plasmid. PCR and Southern blot tests showed that EGFP-prM gene had integrated into C6/36 cell genome. The results indicate that transposon piggyBac element is functional in Ae. albopictus and should be useful in further constructing dengue-refractory transgenic Ae. albopictus. |
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| Keywords: | Aedes albopictus C6/36 cell prM gene piggyBac element transposon |
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