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登革病毒转基因载体pB[PUBnls_EGFP_prM]的构建及其在C6/36细胞中整合作用的检测
引用本文:葛春喜,黄炯烈,陈观今,吴瑜,于洪枫. 登革病毒转基因载体pB[PUBnls_EGFP_prM]的构建及其在C6/36细胞中整合作用的检测[J]. 昆虫学报, 2003, 46(5): 549-552
作者姓名:葛春喜  黄炯烈  陈观今  吴瑜  于洪枫
作者单位:中山大学中山医学院病原生物学教研室,广州,510080
基金项目:“2 11工程”重点学科建设基金资助项目 ( 9812 4)
摘    要:利用转基因技术来探索新的蚊媒疾病防治方法,将登革病毒前膜蛋白基因prM重组入以转座子piggyBac因子为基础的载体,构建了昆虫转基因载体pB[PUBnls-EGFP-prM],在辅助质粒的作用下共同转染白纹伊蚊Aedes albopictus C6/36细胞。PCR和Southern blot证明构建的转基因载体可以将EGFP-prM基因整合入蚊虫基因组中。验证了转座子piggyBac因子、启动子polyubiquitin可以在白纹伊蚊中发挥功能,为进一步构建不传播登革病毒的转基因白纹伊蚊奠定了基础。

关 键 词:白纹伊蚊C6/36细胞   prM基因   piggyBac因子   转座子  
文章编号:0454-6296(2003)05-0549-04
修稿时间:2002-07-24

Construction of transgenic vector pB [PUBnls-EGFP-prM] and identification of its integrational function in mosquito cell
GE Chun-Xi,HUANG Jiong-Lie,CHEN Guan-Jin,WU Yu ,YU Hong-Feng. Construction of transgenic vector pB [PUBnls-EGFP-prM] and identification of its integrational function in mosquito cell[J]. Acta Entomologica Sinica, 2003, 46(5): 549-552
Authors:GE Chun-Xi  HUANG Jiong-Lie  CHEN Guan-Jin  WU Yu   YU Hong-Feng
Affiliation:GE Chun-Xi,HUANG Jiong-Lie,CHEN Guan-Jin,WU Yu *,YU Hong-Feng
Abstract:To explore a new method to control arthropod-borne diseases, prM gene from dengue virus was inserted into a transposon piggyBac based plasmid and an insect transgenic vector pB[PUBnls-EGFP-prM] was constructed. The vector was used to transfect Aedes albopictus C6/36 cell with a helper plasmid. PCR and Southern blot tests showed that EGFP-prM gene had integrated into C6/36 cell genome. The results indicate that transposon piggyBac element is functional in Ae. albopictus and should be useful in further constructing dengue-refractory transgenic Ae. albopictus.
Keywords:Aedes albopictus C6/36 cell  prM gene  piggyBac element  transposon
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