N-terminal sequence analysis of human placental protein 14, purified in high yield from decidual cytosol |
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| Authors: | O M Westwood M G Chapman N Totty R Philp A E Bolton N R Lazarus |
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| Institution: | Department of Obstetrics & Gynaecology, Guy's Hospital, London, U.K. |
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| Abstract: | Human placental protein 14 (PP14) has been purified in high yield from first trimester decidual cytosol. High-performance liquid chromatography on anion exchange, gel filtration and reverse-phase chromatography were used. The protein obtained is approximately 97% pure with an overall recovery of about 50% from the original tissue extract. The first 24 amino acids of the N-terminal were found to be Met-Asp-Ile-Pro-Gln-Thr-Lys-Gln-Asp-Leu-Glu-Leu-Pro-Lys-Leu-Ala-Gly-Thr-Glu-His - Glu-Met-Ala-Met. PP14 has been characterized in this study to be a dimeric glycoprotein of Mr 60,000, with homologous subunits having an Mr of 28,000. |
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