Cyclic GMP and cyclic AMP changes in response to folic acid pulses during cell development of Dictyostelium discoideum. |
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| Authors: | B Wurster K Schubiger P Brachet |
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| Affiliation: | 1. Department of Nutrition, School of Public Health, Guangdong Medical University, No.1 Xincheng Avenue, Songshan Lake Science & Technologic Industry Park, Dongguan, China;2. Dongguan Key Laboratory of Chronic Inflammatory Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical University, No. 42 Jiaoping Road, Tangxia Town, Dongguan, China;1. MOE Laboratory of Biosystems Homeostasis & Protection & College of Life Sciences-iCell Biotechnology Regenerative Biomedicine Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang Province, PR China;2. College of Traditional Chinese Medicine and Health Industry, Lishui University, Lishui 323000, Zhejiang Province, PR China;3. Eye Center, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang Provincial Key Laboratory of Ophthalmology, Zhejiang Provincial Clinical Research Center for Eye Diseases, Zhejiang Provincial Engineering Institute on Eye Diseases, Hangzhou 310009, Zhejiang Province, PR China;4. School of Pharmaceutical Sciences, Hangzhou Medical College, Hangzhou 310013, Zhejiang Province, PR China;1. Departamento de Biología de Sistemas, Universidad de Alcalá, 28871 Alcala de Henares, Madrid, Spain;2. Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034 Madrid, Spain;3. Cellular Biology in Renal Diseases Laboratory, IIS-Fundación Jiménez Díaz-Universidad Autónoma Madrid, 28040 Madrid, Spain;4. Functional Proteomics Laboratory, Centro Nacional de Biotecnología, CSIC, Calle Darwin 3, Campus de Cantoblanco, 28049 Madrid, Spain;5. Departamento de Genética, Fisiología y Microbiología, Universidad Complutense, 28040 Madrid, Spain;6. Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 28040 Madrid, Spain;7. Electron Microscopy Unit, Centro Nacional de Biotecnología, CSIC, Calle Darwin 3, Campus de Cantoblanco, 28049 Madrid, Spain;8. Departamento de Biomedicina y Biotecnología, Universidad de Alcalá, 28871 Alcala de Henares, Madrid, Spain |
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| Abstract: | Folic acid pulses induced developmental processes in agip 71, a morphogenetic mutant of Dictyostelium discoideum, strain Ax-2. Cells that had received folic acid pulses were able to form EDTA-stable cell aggregates and to complete full differentiation to fruiting bodies. In these cells no autonomous periodic activities were observed by light scattering. Folic acid pulses elicited increases in the concentrations of cyclic GMP and cyclic AMP. In undifferentiated cells, folic acid caused a rapid increase in the level of cyclic GMP without a significant change in the level of cyclic AMP. In an advanced developmental state folic acid caused an increase in cyclic AMP in addition to two successsive peaks of cyclic GMP. Experiments performed with the parent strain, Ax-2, also showed that during the development towards aggregation competence, cells acquired the ability to produce a cyclic AMP peak in response to folic acid. |
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