| 大肠杆菌热稳定性肠毒素的分离纯化和氨基酸顺序测定 |
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| 引用本文： | 赵彪,戚正武,承炎,谢梅雯,司马蕙兰.大肠杆菌热稳定性肠毒素的分离纯化和氨基酸顺序测定[J].中国生物化学与分子生物学报,1986,2(5):41-47. |
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| 作者姓名： | 赵彪 戚正武 承炎 谢梅雯 司马蕙兰 |
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| 作者单位： | 中国科学院上海脑研究所
(赵彪)，中国科学院上海生物化学研究所
(戚正武)，上海市卫生防疫站
(承炎，谢梅雯)，上海市卫生防疫站(司马蕙兰) |
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| 摘 要： | 上海市儿童医院婴儿腹泻病患中分离到产肠毒素的菌株,该菌株只产热稳定性肠毒素,经CAD-44树脂吸附,SephadexG-25凝胶过泸,DEAE-Seharose CL-6B离子交换层析,反相高压液相层析分离纯化后,用手工固相DABITC/PITC双偶合Edman降解法测定其氨基酸顺序为:Asn·Ser·Ser·Asn·Tyr·Cys·Cys·Gly·Leu·Cys·Cys·Asn·Pro·Ala·Cys·Thr·Gly·Cys·Tyr。
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| 关 键 词： | 大肠杆菌 肠毒素 肽类毒素 顺序测定 |
| 收稿时间： | 1986-10-20 |
PURIFICATION AND SEQUENCE DETERMINATION OF A HEAT-STABLE PEPTIDE ENTEROTOXIN OF E. COLI |
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| Zhao,Biao.PURIFICATION AND SEQUENCE DETERMINATION OF A HEAT-STABLE PEPTIDE ENTEROTOXIN OF E. COLI[J].Chinese Journal of Biochemistry and Molecular Biology,1986,2(5):41-47. |
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| Authors: | Zhao Biao |
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| Institution: | (Shanghai Brain Research Institute,Academia Sinica)Chi,Cheng-wu(Shanghai Institute of Biochemistry,Academia Sinica) Chen,Yan Xie,Min-wen Sima,Hui-lan (Shanghai Hygiene and Antiepidemic Center |
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| Abstract: | An enterotoxigenic strain of E.coli has been isolated from an infant patient in Shanghai Children Hospital and its heat-stable enteroxoxin has been purified to-homogeneity.The purification scheme involved CAD-44 resin absorption,Sephadex G-25 gel filtration,DEAE-Sephrose CL-6B ion exchange chromatography,and re-verse phase high performance liquid chromatography.The amino acid sequence of the purified toxin was determined by manual DABITC/PITC double-coupling solid-phase Edman degradation to be Asn-Ser-Ser-Asn-Tyr-Cys-Cys-Glu-Leu-Cys-Cys-Asn-Pro-Ala-Cys-Thr-Gly-Cys-Tyr. |
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