| 尿枸橼酸盐全自动酶法测定及临床应用 |
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| 引用本文： | 张瑞生,高应东,周拥军. 尿枸橼酸盐全自动酶法测定及临床应用[J]. 现代生物医学进展, 2010, 10(5) |
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| 作者姓名： | 张瑞生 高应东 周拥军 |
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| 作者单位： | 南京医科大学附属南京第一医院检验科,江苏,南京,210006 |
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| 摘 要： | 目的:建立全自动生化分析仪测定尿枸橼酸盐的方法,探讨其在枸橼酸钾治疗泌尿系结石患者中的应用。方法:采用酶偶联速率法,观察重复性、线性及回收实验,并测定36例泌尿系结石病人口服枸橼酸钾片前及服药后24小时尿枸橼酸、尿钾和尿钙。结果:该法重复性CV分别为2.26%和3.34%,枸橼酸浓度在5mmol/L的范围内呈线性,r为0.9981。服药后,尿枸橼酸明显升高(p<0.05),尿钙显著降低(p<0.05)。结论:使用全自动酶法测定尿枸橼酸盐重复性好,准确度高,口服枸橼酸钾能碱化尿液,降低尿钙,起到防治泌尿系结石的作用。
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| 关 键 词： | 全自动生化分析仪 枸橼酸钾 泌尿系结石 |
Determination of urinary citrate by automatic enzyme and its clinical application |
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| zhang Rui-sheng,Gao Ying-dong,Zhou Yong-jun. Determination of urinary citrate by automatic enzyme and its clinical application[J]. Progress in Modern Biomedicine, 2010, 10(5) |
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| Authors: | zhang Rui-sheng Gao Ying-dong Zhou Yong-jun |
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| Affiliation: | Zhang Rui-sheng,Gao Ying-dong,Zhou Yong-jun (Inspection division,Nanjing first hospital affiliated to Najing Medical University,Nanjing 210006,China) |
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| Abstract: | Objective:To establish the determination method of the urinary citrate by automatic biochemical analyzer,and explore the application of potassium citrate to treat patients with urinary stone.Method:Repeatability,linearity and recovery experiment were detected by the method of enzyme coupling rate,and urinary citrate,urinary potassium and calcium were determined in patients administrated with oral potassium citrate tablets before and after 24 hours.Results:Repeated CV of the method was 2.26% and 3.34%,and th... |
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| Keywords: | Automatic biochemical analyzer Potassium citrate Urinary stone |
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