Factors affecting somatic embryogenesis in <Emphasis Type="Italic">Prunus incisa</Emphasis> cv. February Pink |
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Authors: | E?J?Cheong Email author" target="_blank">M?R?PoolerEmail author |
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Institution: | (1) Jeju Forest Experiment Station, Korea Forest Research Institute, 1253 Sanghyodong, Seogwipo, Jeju, 697-050, South Korea;(2) Floral and Nursery Plants Research Unit, USDA/ARS, US National Arboretum, 3501 New York Ave. NE, Washington, D.C. 20002, USA |
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Abstract: | Factors affecting somatic embryogenesis from root explants of Prunus incisa Thunb. cv. February Pink were investigated. Using a medium containing Murashige and Skoog salts and vitamins supplemented with 10 M 2,4-dichlorophenoxyacetic (2,4-D), we evaluated the effects of light, growth regulators, amino acids, carbohydrate source, and root induction medium. Explants cultured under light or dark conditions both resulted in the formation of embryos. Embryogenesis was inhibited by the addition of 6-benzyladenine, thidiazuron, or gibberellic acid to the medium. Amino acids were not effective in promoting embryogenesis, with high levels of amino acids actually inhibiting it. Sucrose and glucose effectively induced embryogenesis, while sorbitol and mannitol completely inhibited it. Sucrose and glucose also promoted secondary embryogenesis. Embryos that formed in medium containing 4% or 5% sucrose were abnormally shaped and did not fully develop, while those that formed in medium with sucrose concentrations of 2% or 3% were much more vigorous. Root explants that were induced on medium containing 1.0 M indole-3-butyric acid (IBA) produced more somatic embryos than explants induced on medium without IBA. Approximately 50% of the roots induced on medium containing 1.0 M IBA produced somatic embryos on medium containing 10 M 2,4-D and 3% sucrose.Abbreviations BA 6-Benzyladenine - 2,4-D 2,4-Dichlorophenoxyacetic acid - GA3 Gibberellic acid - IBA Indole-3-butyric acid - NAA -Naphthaleneacetic acid - TDZ Thidiazuron |
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Keywords: | Prunus incisa Flowering cherry Somatic embryogenesis Nursery crops |
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