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影响体胚发生途径香蕉(Musa spp.,AAB Group)植株再生的因素
引用本文:徐春香,Bart PANIS,Rony SWENNEN,李华平,肖火根,范怀忠.影响体胚发生途径香蕉(Musa spp.,AAB Group)植株再生的因素[J].植物生理学通讯,2004,40(3):293-296.
作者姓名:徐春香  Bart PANIS  Rony SWENNEN  李华平  肖火根  范怀忠
作者单位:1. 华南农业大学园艺学院热带亚热带果树研究室
2. 比利时荷语鲁汶大学热带作物改良实验室,比利时鲁汶B-3001
3. 华南农业大学植物病毒研究室,广州,510642
基金项目:国家自然科学基金,广东省自然科学基金,广东省科技攻关项目
摘    要:香蕉品种‘Agbagaba'和‘Orishele'的胚性细胞悬浮系(ECS)在液体培养基中分别预培养1和2周后,将其接种在RD1和M3培养基上,于光照或黑暗条件下进行体胚的再生.从沉积细胞体积(SCV)为1 mL(1 mL SCV)的ECS获得的再生体胚数量因预培养时间、再生培养基的种类及培养条件的不同而异.植株的再生率及从1 mL SCV的ECS获得的再生植株数量也受上述体胚再生条件的间接影响.

关 键 词:香蕉(Musa  spp.  AAB  Group)  体胚发生  体胚再生条件  植株再生
修稿时间:2003年7月18日

Factors Affecting Banana (Musa spp.,AAB Group) Plant Regeneration via Embryogenesis
Bart PANIS,Hannelore STROSSE,Rony SWENNEN.Factors Affecting Banana (Musa spp.,AAB Group) Plant Regeneration via Embryogenesis[J].Plant Physiology Communications,2004,40(3):293-296.
Authors:Bart PANIS  Hannelore STROSSE  Rony SWENNEN
Abstract:Embryogenic cell suspensions(ECSs) of Agbagaba and Orishele (Musa spp., AAB Group) wereused to regenerate embryos after 1-week and 2-week pre-culture in liquid medium respectively. The ECSs wereinoculated on regeneration medium RD1 or M3 and incubated under light or in dark. The amount of regenerablesomatic embryos that could be regenerated from per milliliter settled cell volume (SCV) of ECS depended onpre-culture time in liquid media, the types of regeneration media and incubation conditions. Plant regenerationfrequency from the somatic embryos and plantlets could be regenerated from 1 mL SCV of ECS were indirectlyaffected with those conditions for the regeneration of somatic embryos mentioned above.
Keywords:bananas (Musa spp    AAB Group)  somatic embryogenesis  conditions for the regeneration ofsomatic embryos  regeneration of plantlets
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