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Plasmodium falciparum: continuous cultivation in a semiautomated apparatus.
Authors:J B Jensen  W Trager  J Doherty
Institution:The Rockefeller University, New York, New York 10021, U.S.A.
Abstract:A semi-automated apparatus for the continuous cultivation of the malarial parasite, Plasmodium falciparum, was developed. It changes the culture medium and redistributes ths infected erythrocytes at preselected intervals. Parasitemias between 2 and 16% can be maintained by adding fresh erythrocytes every 2 or 3 days. This apparatus produces approximately 10 ml of packed erythrocytes per week with parasitemias between 12 and 16%
Keywords:Protozoa  parasitic  Malaria  Continuous culture
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