Specificity and timing of the Zn2+ requirement for DNA synthesis by 3T3 cells |
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Authors: | J K Chesters L Petrie H Vint |
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Affiliation: | Rowett Research Institute, Bucksburn, Aberdeen, United Kingdom. |
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Abstract: | Addition of the chelator DTPA to synchronized cultures of 3T3 cells inhibited thymidine incorporation by up to 90% and only Zn2+ of the divalent cations tested was effective in reversing this effect. Fe2+ given alone had no effect on the inhibition by DTPA but when added to Zn2+ supplemented cultures increased thymidine incorporation from approximately 80-90 to over 100% of that in control cultures. Investigations indicated that the major requirement for Zn2+ was within the period from 8 h after stimulation of quiescent cells with serum until 3 h before the start of S phase. There was also an indication of a further requirement for Zn2+ at the G/S transition. |
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