α-Galactosidase Aga27A, an Enzymatic Component of the Clostridium josui Cellulosome |
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Authors: | Sadanari Jindou Shuichi Karita Emi Fujino Tsuchiyoshi Fujino Hidenori Hayashi Tetsuya Kimura Kazuo Sakka and Kunio Ohmiya |
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Institution: | Faculty of Bioresources, Mie University, Tsu 514-8507, Japan. |
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Abstract: | The Clostridium josui aga27A gene encodes the cellulosomal alpha-galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various alpha-galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms, especially plants. The recombinant Aga27A alpha-galactosidase devoid of the dockerin domain preferred highly polymeric galactomannan as a substrate to small saccharides such as melibiose and raffinose. |
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