Development of stable reporter system cloning luxCDABE genes into chromosome of Salmonella enterica serotypes using Tn7 transposon |
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| Authors: | Kevin Howe Attila Karsi Pierre Germon Robert W Wills Mark L Lawrence Richard H Bailey |
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| Affiliation: | 1.Department of Pathobiology and Population Medicine, College of Veterinary Medicine,Mississippi State University,Mississippi State,USA;2.Department of Basic Sciences,College of Veterinary Medicine, Mississippi State University,Mississippi State,USA;3.Institute for Digital Biology,Mississippi State University,Mississippi State,USA;4.INRA, UR 1282 Infectiologie Animale et Santé Publique,Laboratoire de Pathogénie Bactérienne,Nouzilly,France |
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| Abstract: | Background Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent Salmonella enterica serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of luxCDABE genes into the chromosome of eleven Salmonella enterica serotypes isolated from the broiler production continuum. |
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