首页 | 本学科首页   官方微博 | 高级检索  
     


Development of stable reporter system cloning luxCDABE genes into chromosome of Salmonella enterica serotypes using Tn7 transposon
Authors:Kevin Howe  Attila Karsi  Pierre Germon  Robert W Wills  Mark L Lawrence  Richard H Bailey
Affiliation:1.Department of Pathobiology and Population Medicine, College of Veterinary Medicine,Mississippi State University,Mississippi State,USA;2.Department of Basic Sciences,College of Veterinary Medicine, Mississippi State University,Mississippi State,USA;3.Institute for Digital Biology,Mississippi State University,Mississippi State,USA;4.INRA, UR 1282 Infectiologie Animale et Santé Publique,Laboratoire de Pathogénie Bactérienne,Nouzilly,France
Abstract:

Background  

Salmonellosis may be a food safety problem when raw food products are mishandled and not fully cooked. In previous work, we developed bioluminescent Salmonella enterica serotypes using a plasmid-based reporting system that can be used for real-time monitoring of the pathogen's growth on food products in short term studies. In this study, we report the use of a Tn7-based transposon system for subcloning of luxCDABE genes into the chromosome of eleven Salmonella enterica serotypes isolated from the broiler production continuum.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号