Bifunctional protein conferring enhanced green fluorescence and puromycin resistance |
| |
Authors: | Abbate J Lacayo J C Prichard M Pari G McVoy M A |
| |
Institution: | Governor's School for Government and International Studies, Richmond, VA, USA. |
| |
Abstract: | A new genetic marker was created in which sequences from enhanced green fluorescent protein were fused to those of puromycin N-acetyl transferase. The resulting fusion protein (EGFP-puro) conferred both green fluorescence and resistance to puromycin when expressed in mammalian cells. The utility of EGFP-puro as a selectable/screenable marker was demonstrated by the ease with which a recombinant guinea pig cytomegalovirus containing EGFP-puro was isolated by a combination of puromycin selection and screening for green fluorescence. We conclude that EGFP-puro is a compact and versatile marker that should prove useful for recombinant virus and transgenic cell line construction, particularly in applications in which coding capacity is limited. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|