Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase |
| |
Authors: | Watanabe Seiya Saleh Ahmed Abu Pack Seung Pil Annaluru Narayana Kodaki Tsutomu Makino Keisuke |
| |
Affiliation: | Faculty of Engineering, Kyoto University, Kyotodaigaku-katsura, Kyoto 615-8530, Japan. irab@iae.kyoto-u.ac.jp |
| |
Abstract: | Effects of reversal coenzyme specificity toward NADP+ and thermostabilization of xylitol dehydrogenase (XDH) from Pichia stipitis on fermentation of xylose to ethanol were estimated using a recombinant Saccharomyces cerevisiae expressing together with a native xylose reductase from P. stipitis. The mutated XDHs performed the similar enzyme properties in S. cerevisiae cells, compared with those in vitro. The significant enhancement(s) was found in Y-ARSdR strain, in which NADP+-dependent XDH was expressed; 86% decrease of unfavorable xylitol excretion with 41% increased ethanol production, when compared with the reference strain expressing the wild-type XDH. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|