The Slice Culture Method for Following Development of Tooth Germs In Explant Culture |
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Authors: | Sarah A. Alfaqeeh Abigail S. Tucker |
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Affiliation: | 1.Department of Craniofacial Development and Stem Cell Biology, and Department of Orthodontics, Dental Institute, Guy''s Hospital, UK, King''s College London;2.Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Kingdom of Saudi Arabia |
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Abstract: | Explant culture allows manipulation of developing organs at specific time points and is therefore an important method for the developmental biologist. For many organs it is difficult to access developing tissue to allow monitoring during ex vivo culture. The slice culture method allows access to tissue so that morphogenetic movements can be followed and specific cell populations can be targeted for manipulation or lineage tracing.In this paper we describe a method of slice culture that has been very successful for culture of tooth germs in a range of species. The method provides excellent access to the tooth germs, which develop at a similar rate to that observed in vivo, surrounded by the other jaw tissues. This allows tissue interactions between the tooth and surrounding tissue to be monitored. Although this paper concentrates on tooth germs, the same protocol can be applied to follow development of a number of other organs, such as salivary glands, Meckel''s cartilage, nasal glands, tongue, and ear. |
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Keywords: | Anatomy Issue 81 Tooth Culture Techniques Embryo Culture Techniques Organ Culture Techniques Developmental Biology animal biology animal models Tooth germ live slice development tissue chopper lineage tracing molar incisor gland |
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