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Noninvasive Intratracheal Intubation to Study the Pathology and Physiology of Mouse Lung
Authors:Yan Cai  Shioko Kimura
Affiliation:1.Laboratory of Metabolism, National Cancer Institute, National Institutes of Health
Abstract:The use of a model that mimics the condition of lung diseases in humans is critical for studying the pathophysiology and/or etiology of a particular disease and for developing therapeutic intervention. With the increasing availability of knockout and transgenic derivatives, together with a vast amount of genetic information, mice provide one of the best models to study the molecular mechanisms underlying the pathology and physiology of lung diseases. Inhalation, intranasal instillation, intratracheal instillation, and intratracheal intubation are the most widely used techniques by a number of investigators to administer materials of interest to mouse lungs. There are pros and cons for each technique depending on the goals of a study. Here a noninvasive intratracheal intubation method that can directly deliver exogenous materials to mouse lungs is presented. This technique was applied to administer bleomycin to mouse lungs as a model to study pulmonary fibrosis.
Keywords:Medicine   Issue 81   mouse   rodents   intratracheal intubation   delivery of exogenous substances   lung   study of airway pathology and physiology   pulmonary fibrosis
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