| 两种A类氨基酸转运蛋白部分功能差异的研究 |
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| 引用本文： | 明洪,龙莉,曾惠芳,李京昆,张闻. 两种A类氨基酸转运蛋白部分功能差异的研究[J]. 细胞生物学杂志, 2003, 25(2): 104-108 |
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| 作者姓名： | 明洪 龙莉 曾惠芳 李京昆 张闻 |
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| 作者单位： | 昆明医学院生物学教研室昆明650031 |
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| 摘 要： | 应用重组Vaccinia病毒表达系统在猴肾成纤维细胞CV—1中瞬时表达大鼠A类氨基酸转运蛋白GlnT和SAT—2，研究它们转运MeAIB、Ala和Gln的动力学和对多种氨基酸转运亲和性的差异，结合它们的氨基酸序列同源性比较表明：虽然GlnT和SALT—2蛋白的氨基酸序列有较高的同源性，但它们在转运功能上有较大的差异。
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| 关 键 词： | A类氨基酸转运蛋白 GlnT SAT－2 转运动力学 转运亲和性 转运功能 重组Vaccinia病毒表达系统 |
| 修稿时间： | 2001-12-10 |
STUDY ON THE FUNCTIONAL DIFFERENCE BETWEEN TWO SUBTYPES OF AMINO ACID TRANSPOTER SYSTEMA |
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| MING Hong LONG Li ZENGHui Fang LI Jing Kun ZHANG Wen. STUDY ON THE FUNCTIONAL DIFFERENCE BETWEEN TWO SUBTYPES OF AMINO ACID TRANSPOTER SYSTEMA[J]. Chinese Journal of Cell Biology, 2003, 25(2): 104-108 |
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| Authors: | MING Hong LONG Li ZENGHui Fang LI Jing Kun ZHANG Wen |
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| Abstract: | By transient expressing of rat system A amino acid transporters Gln T and STA-2 in monkey fibroblast cell line CV-1 with recombination vaccinia virus expression system, we studied affinity of transporting some amino acids and kinetics of transporting MeAIB, alanine and glutamine by Gln T and SAT-2. Combined with analysis of their amino acid sequences, it suggested that Gln T and SAT-2 played different roles in amino acid transporting in spite of their higher homology in amino acid sequence. |
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| Keywords: | System A amino acid transporter Recombination vaccinia virus CV-1 Gln TSAT-2 Rat |
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