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Release of aminopeptidase from Lactobacillus casei sp. casei by cell disruption in a Microfluidizer
Authors:H. Choi  L. Laleye  G.F. Amantea  R.E. Simard
Affiliation:(1) Research and Development, Ault Foods Limited, 75A Bathurst Street, London, Ontario, Canada, N6B 1N8;(2) Faculté des Sciences de L"lsquo"agriculture et de L"rsquo"alimentation, Université Laval, Québec, Canada, G1K 7P4
Abstract:Cell disruption in a Microfluidizer was a function of both operating pressure and number of passes. The operating pressure had greater effect on the disruption than did the number of passes as indicated by the magnitude of the constants from the cell disruption equation. Protein release correlated with aminopeptidase release by Lactobacillus casei sp. casei. The optimum operating pressure for enzyme extraction was 76 MPa with loss of enzyme activity about 15 to 20%.
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