Outer membrane nanovesicles of gram-negative bacteria <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis> and <Emphasis Type="Italic">Aeromonas salmonicida</Emphasis> |
| |
Authors: | K A Lusta Yu E Kozlovskii |
| |
Affiliation: | 1.Scientific Research Institute of Human Morphology,Russian Academy of Medical Sciences,Moscow,Russia |
| |
Abstract: | Aeromonas hydrophila and A. salmonicida grown in pure cultures were found to secrete extracellular membrane nanovesicles into the environment. Outer membrane nanovesicle
preparations were isolated by differential centrifugation and ultrafiltration and visualized by transmission electron microscopy
applying the negative staining technique. Membrane nanovesicle size (10–300 nm) and ultrastructure were determined. The vesicles
were especially numerous around bacteria at the edge of small colonies. The process of the vesicle budding off the bacterial
cell was observed. On ultrathin sections of rat intestine, outer membrane nanovesicles were revealed among bacterial aggregates
of various species of parietal microorganisms. Short chains of such vesicles were also detected inside the glycocalyx between
the microvilli of the apical surface of the intestine epitheliocytes. On the basis of the results, together with the literature
data, the secretion of the outer membrane nanovesicles by pathogenic gram-negative bacteria, such as A. hydrophila and A. salmonicida, is proposed as a possible mechanism of pathogenesis leading to the host disease, as well as a means for cellular interactions
both within the prokaryote population and between the bacteria and the host organism. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|