| 不吸水链霉菌11371衍生菌种构建及基因工程宿主菌初筛 |
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| 引用本文： | 陈飞,张璧,吴红艳,桓明辉,关艳丽,郭玲玲.不吸水链霉菌11371衍生菌种构建及基因工程宿主菌初筛[J].生物技术通报,2009(1). |
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| 作者姓名： | 陈飞 张璧 吴红艳 桓明辉 关艳丽 郭玲玲 |
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| 作者单位： | 辽宁省微生物科学研究院,朝阳,122000 |
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| 摘 要： | 采用物理、化学等方法对不吸水链霉菌11371(Streptomyces ahygroscopicus wuzhouensis subsp)进行诱变,获得78株在菌落形态方面与野生菌不同的衍生菌;并对各种衍生菌株进行了抗生素生物活性、内源性质粒以及外源载体转化等研究,初步筛选出2株具有宿主潜力的衍生菌,为构建11371转化体系提供了生物材料和相关理论基础。
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| 关 键 词： | 不吸水链霉菌 基因工程宿主 |
Establishment of Strain Derived from 11371 and Screen of Gene Host System |
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| Chen Fei,Zhang Bi,Wu Hongyan,Huan Minghui,Guan Yanli,Guo Lingling.Establishment of Strain Derived from 11371 and Screen of Gene Host System[J].Biotechnology Bulletin,2009(1). |
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| Authors: | Chen Fei Zhang Bi Wu Hongyan Huan Minghui Guan Yanli Guo Lingling |
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| Abstract: | This method of physics and chemistry was adopted to induce Streptomyces ahygroscopicus wuzhouensis subsp. 11371,and 78 Strain derived from 11371 were gained. 2 Gene Host were firstly Screened through studiing avidity,plasmids and vector transform. The foundation of boil-material and relative theory was establish to study 11371 Gene Host System. |
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| Keywords: | Streptomyces ahygroscopicus Gene host system |
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