Synergistic Effect of Selenium and Melatonin on Neuroprotection in Cerebral Ischemia in Rats |
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Authors: | Ajmal Ahmad Mohd Moshahid Khan Tauheed Ishrat M Badruzzaman Khan Gulrana Khuwaja Syed Shadab Raza Pallavi Shrivastava Fakhrul Islam |
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Institution: | (1) Department of Internal Medicine, Carver College of Medicine, 500 Newton Road University of Iowa, Iowa city, IA 52242, USA;(2) Brain Research Laboratory, Department of Emergency Medicine, Emory University, Atlanta, GA 30322, USA;(3) Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA 30912, USA;(4) Neurotoxicology laboratory, Department of Medical Elementology & Toxicology, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi, 110062, India; |
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Abstract: | The synergistic scavenger effects of selenium and melatonin collectively we called Se-Mel was studied on the prevention of
neuronal injury induced by ischemia/reperfusion. Male Wistar rats were treated with sodium selenite (0.1 mg/kg, i.p.) and
melatonin (10 mg/kg, i.p.) 30 min before the middle carotid artery occlusion (MCAO) and immediately after MCAO to male Wistar
rats and was continued for 3 days once daily at the interval of 24 h. Behavioral activity (spontaneous motor activity and
motor deficit) was improved in Se-Mel-treated rats as compared to MCAO group rats. The level of glutathione and the activity
of antioxidant enzymes was depleted significantly while the content of thiobarbituric acid reactive substances, protein carbonyl,
and nitric oxide radical (NO·) was increased significantly in MCAO group. Systemic administration of Se-Mel ameliorated oxidative stress and improves ischemia/reperfusion-induced
focal cerebral ischemia. Se-Mel also inhibited inducible nitric oxide synthase expression in Se-Mel+MCAO group as compared
to MCAO group rats. Thus, Se-Mel has shown an excellent neuroprotective effect against ischemia/reperfusion injury through
an anti-ischemic pathway. In conclusion, we demonstrated that the pretreatment with Se-Mel at the onset of reperfusion, reduced
post-ischemic damage, and improved neurological outcome following transient focal cerebral ischemia in male Wistar rat. |
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