芳香族化合物生物降解的研究进展

本文综述了以苯、取代苯、联苯和多环芳烃为代表的芳香族化合物的生物降解途径,其共同之处在于经过两步双加氧酶作用,生成二醇和开环。两步双加氧酶分别为芳环羟基化双加氧酶和芳环断裂双加氧酶。以甲苯途径为代表讨论了芳香族化合物的分子生物学研究情况。代谢工程研究是九十年代兴起的芳香族化合物生物降解的研究内容,通过对甲苯途径的代谢工程研究明确了途径中的关键酶,并通过对关键酶的活性提高使整个途径的代谢流增加。

Advance on the Studies of Biodegradation of Aromatic compounds

Biodegradation of aromatic compounds such as benzene\,alkylbenzenes\,biphenyl and polycyclic aromatic hydrocarbons is reviewed.The conversion of them to cis \|diols and the ring\|cleavage by two kinds of dioxygenases are all involved in the pathway.The molecular biology research about the TOL\|pathway is also discussed here.Metabolic engineering is introduced into the aromatic compounds degradation in early 90's.In the pathway,rate\|controlling enzyme can be determined and the flux through the pathway increased by increasing the titer of the rate\|controlling enzyme.