Behavioural assessments of neurotoxic effects and neurodegeneration in zebrafish |
| |
Authors: | Keith B. Tierney |
| |
Affiliation: | Department of Biological Sciences, University of Alberta, Z 718 Biological Sciences Building, Edmonton, Alberta, Canada T6G 2E9 |
| |
Abstract: | Altered neurological function will generally be behaviourally apparent. Many of the behavioural models pioneered in mammalian models are portable to zebrafish. Tests are available to capture alterations in basic motor function, changes associated with exteroceptive and interoceptive sensory cues, and alterations in learning and memory performance. Excepting some endpoints involving learning, behavioural tests can be carried out at 4 days post fertilization. Given larvae can be reared quickly and in large numbers, and that software solutions are readily available from multiple vendors to automatically test behavioural responses in 96 larvae simultaneously, zebrafish are a potent and rapid model for screening neurological impairments. Coupling current and emerging behavioural endpoints with molecular techniques will permit and accelerate the determination of the mechanisms behind neurotoxicity and degeneration, as well as provide numerous means to test remedial drugs and other therapies. The emphasis of this review is to highlight unexplored/underutilized behavioural assays for future studies. This article is part of a Special Issue entitled Zebrafish Models of Neurological Diseases. |
| |
Keywords: | AChE, acetylcholinesterase Anti-AChE, anticholinesterase CPP, conditioned place preference CS, conditioned stimulus dpf, days post fertilization OKR, optokinetic response OMR, optomotor response OP, organophosphorus agent OSN, olfactory sensory neuron PEA, phenylethyl alcohol PTZ, pentylenetetrazole UCS, unconditioned stimulus |
本文献已被 ScienceDirect 等数据库收录! |
|