Isolation and characterisation of a sucrose: sucrose 1-fructosyltransferase gene from perennial ryegrass (Lolium perenne) |
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| Authors: | Chalmers Jaye Johnson Xenie Lidgett Angela Spangenberg German |
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| Affiliation: | Plant Biotechnology Centre, Agriculture Victoria, Department of Primary Industries, CRC for Molecular Plant Breeding, La Trobe University, Bundoora, Victoria 3086, Australia. |
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| Abstract: | A sucrose: sucrose 1-fructosyltransferase (1-SST) gene and cDNA (Lp 1-SST) from perennial ryegrass (Lolium perenne) were isolated. The Lp 1-SST gene was fully sequenced and shown to contain three exons and two introns. Nucleotide sequence analysis of the 4824 bp Lp 1-SST genomic sequence revealed 1618 bp of 5' UTR and an open reading frame of 1962 bp encoding a protein of 653 amino acids. Lp 1-SST is 95% identical to the tall fescue 1-SST and contains plant fructosyltransferase functional domains. Lp 1-SST corresponds to a single copy gene in perennial ryegrass, and is expressed in young leaf bases and mature leaf sheaths. The recombinant Lp 1-SST protein from corresponding cDNA expression in Pichia pastoris showed 1-SST activity. |
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| Keywords: | fructan biosynthesis fructosyltransferase Lolium perenne perennial ryegrass sucrose : sucrose 1-fructosyltransferase |
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