Chondrocyte apoptosis enhanced at the growth plate: a physeal response to a diaphyseal fracture |
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Authors: | Sonja Gaber Eva Elisa Fischerauer Eleonore Fröhlich Gregor Janezic Florian Amerstorfer Annelie-Martina Weinberg |
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Institution: | (1) Medical University of Graz, Clinic of Paediatric Surgery, Auenbruggerplatz 34, 8036 Graz, Austria |
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Abstract: | Post-traumatic overgrowth of growing long bones is a common clinical phenomenon in paediatric traumatology and is the result
of an enhanced stimulation of the nearby growth plate after fracture. To date, the exact post-fractural reactions of the growth
plate are poorly understood. The aim of this study has been to determine the impact of fracture on the frequency of chondrocyte
apoptosis of the growth plate. Rats sustained a mid-diaphyseal closed fracture of the left tibia or were left untreated. All
animals were killed 3, 10, 14 or 29 days after trauma. The left and right tibiae were harvested and apoptotic chondrocytes
of the proximal tibial growth plate were detected by TUNEL staining. The apoptosis percentage of physeal chondrocytes was
statistically compared among fractured bones, intact contra-lateral bones and control bones. The physeal apoptosis rate of
the fractured bone was significantly higher than that of the contra-lateral intact bone (valid for all evaluated days) and
the control bone (valid from day 10 onwards). Contra-lateral intact tibiae never showed significantly higher apoptosis rates
compared with control tibiae. Thus, mid-diaphyseal fracture influences the nearby growth plate by stimulating chondrocyte
programmed cell death, which is associated with cartilage resorption and bone replacement. The lack of a significant difference
between the intact contra-lateral and the intact control bone suggests that fracture only has a local effect that contributes
to the greater apoptosis rate of the adjacent physis. |
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Keywords: | Apoptosis Chondrocyte Growth plate Fracture TUNEL Rat (Sprague Dawley) |
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