Gibberellin estimation and biosynthesis in germinating Hordeum distichon |
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Authors: | Murphy George Johnstone Park Briggs Dennis Edward |
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Affiliation: | Department of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT U.S.A. |
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Abstract: | Gibberellic acid (GA3) and 13-deoxy-gibberellic acid (GA7) were identified in extracts of germinating barley as their 14C-methyl esters. The maximal level of GA3 was estimated by an isotopic dilution procedure to be 1·5 ng per grain. Germinating barley incorporated 2-14C-mevalonic acid into several terpenes, whose specific radioactivities were measured, but incorporation into GA3 could not be detected. Cell-free embryo extracts from germinating barley converted 2-14C-mevalonic acid into isopentenol, dimethylallyl alcohol, farnesol and squalene, while 14C-isopentenyl pyrophosphate was incorporated into geraniol, farnesol, geranylgeraniol and squalene. There was no detectable incorporation into the gibberellin intermediate ent-kaurene. |
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Keywords: | Gramineae barley germination gibberellins identification biosynthesis. |
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