Callose Synthase in Pinus sylvestris Response during Infection by Species of Heterobasidion annosum sensu lato with Varied Host Preferences |
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Authors: | Marcin Zadworny Dariusz J Smoliński Micha? ?widzinski Marzenna Guzicka Piotr ?akomy Joanna Mucha |
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Institution: | 1. Institute of Dendrology, Polish Academy of Sciences, , 62‐035 Kórnik, Poland;2. Department of Cell Biology, Institute of General and Molecular Biology, Nicolaus Copernicus University, , Toruń, Poland;3. Department of Forest Pathology, Faculty of Forestry, Poznań University of Life Sciences, , Poznań, Poland |
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Abstract: | Strengthening of plant cell walls at the site of fungal entry is one of the earliest plant responses to fungal pathogens. The aim of our study was to characterize the pattern of callose synthase localization and callose deposition in roots of Pinus sylvestris after infection by species of the Heterobasidion annosum s.l. complex with different host specificity: H. annosum s.s., H. parviporum and H. abietinum. To address this, sense‐labelled probes and ribonuclease‐treated samples were used to determine in situ hybridizations of callose synthase by FISH method. Furthermore, determination of callose accumulation within P. sylvestris cells was carried out using aniline blue. The different species of H. annosum s.l. had distinct impacts on the callose synthase staining within plant tissues. Moreover, while inoculation with strains of H. abietinum resulted in callose synthase accumulation at the point of hyphae contact with the host cell, this was not observed with the other species. A significant difference in callose synthesis localization was observed after inoculation with varied species of H. annosum s.l. as a result of the specific interactions with the host. |
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Keywords: | callose synthase host susceptibility necrotrophic pathogen pathogen specificity |
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