Mutagenic activity of ethylenimine in Neurospora crassa |
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Authors: | FJ De Serres |
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Institution: | Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tenn. U.S.A. |
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Abstract: | The mutagenic activity of the monofunctional alkylating agent ethylenimine (EI) was tested with the adenine-3 (ad-3) system in a two-component heterokaryon of Neurospora crassa. The results of forward-mutation experiments showed that EI is a potent mutagen in N. crassa.Genetic analysis of EI-induced ad-3 mutants showed that the frequencies of leakiness, allelic complementation, and non-polarized complementation patterns are similar to those of ad-3 mutants induced by other alkylating agents. It seems, therefore, that in addition to multilocus deletions (which occur at low a frequency), EI-induced mutations probably include base-pair substitutions, frameshift mutations, and other types of intragenic alterations. |
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Keywords: | adenine-3 AP 2-aminopurine colonial temperature sensitive EI ethylenimine EMS ethyl methanesulfonate NA nitrous acid TEM triethylene melamine |
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