Comparison of cell-walls of Lolium multiflorum with cotton cellulose in relation to their digestion with enzymes associated with cellulolysis |
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Authors: | RD Hartley EC Jones TM Wood |
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Institution: | Grassland Research Institute, Hurley, Berks. UK;Rowett Research Institute, Bucksburn, Aberdeen U.K. |
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Abstract: | Activities of several enzymes associated with cellulolysis were compared using as substrates cell-walls of Lolium multiflorum and cotton cellulose. Purified enzymes C1 (see Ref. 1 for definition), C.x (CM-cellulase) and β-glucosidase were employed as well as culture filtrates containing Cx. Activities were determined by ability to digest the substrates and to release H2O-soluble phenolic compounds from the grass cell-walls. The culture filtrates most active on cotton cellulose were obtained using the fungi Trichoderma viride and Fusarium solani; with grass cell-walls the most active were from T. viride, Gliocladium roseum, a species of Basidiomycetes, and one strain of Myrothecium verrucaria (IMI Strain 25 291). For the crude enzyme preparations tested, there were highly significant correlations between the digestibility of grass cell-walls and the UV-absorption of the filtrate at λmax 290 nm and at λmax 324 nm but there was no significant correlation between the digestibility of grass cell-walls and that of cotton cellulose. Partially purified C1 and Cx from two different fungal sources showed activity on both substrates. Differences in MW of the H2O-soluble phenolic compounds obtained by treatment of grass cell-walls with C1 and Cx components suggest that these enzymes could have different modes of action. Synergism between C1 and Cx from T. koningii occurred with both substrates but with C1 and Cx from F. solani synergism only occurred with cotton cellulose. |
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Keywords: | Gramineae cell-walls cellulolysis lignin-carbohydrate linkages ferulic acid |
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