Ribosomal type RNA of rat liver mitochondria

A method for the isolation and purification of rat liver mitochondria in the presence of an extract of natural RNAase inhibitor is described. Mitochondria free from contamination and undegraded mitochondrial ribosomal type RNA are obtained. Sedimentation coefficient measurements and base composition analysis showed significant differences between homologous mitochondrial and cytoplasmic rRNA's. The mitochondrial low molecular weight rRNA showed a higher electrophoretic mobility through 2.4% polyacrylamide gel at different ionic strengths than the homologous cytoplasmic component.