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Gas chromatography-mass spectrometry study of sterols from Pinus elliotti tissues
Authors:John L. Laseter  Robyn Evans  Charles H. Walkinshaw  John D. Weete
Affiliation:Department of Biological Sciences, Louisiana State University in New Orleans, New Orleans, LA 70122, U.S.A.;Lunar Science Institute, 3303 Nasa Road 1, Houston, TX 77058, U.S.A.
Abstract:A comparative study of the sterol components of slash pine (Pinus elliottii) callus tissue cultures, seeds, and seedlings was carried out using GC-MS techniques. Cholesterol, desmosterol, campesterol, stigmasterol, sitosterol and cycloeucalenol were identified in all tissues while lophenol and 24-methylenelophenol were identified in only the seed and seedlings. 24-Ethylidenelophenol was detected in trace concentrations in only the seedlings. Sitosterol was the predominant sterol component, i.e. 80·8, 38·1 and 47·8% of the tissue culture, seed and seedling sterols, respectively.
Keywords:Pinaceae  slash pine  sterols  tissue culture.
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