Removal of the acetate-moiety of 2,4-dichlorophenoxyacetic acid in Ribes sativum |
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Authors: | JR Fleeker |
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Institution: | Department of Biochemistry, North Dakota State University, Fargo, ND 58102, U.S.A. |
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Abstract: | Ten minutes after uptake of 2,4-dichlorophenoxyacetic acid-1-14C(2,4-D-1-14C) by excised Ribes sativum leaves, 37·8 % of the radioactivity in water-soluble metabolites was in glyoxylic acid. When 2,4-D- 2-14C was supplied under the same conditions, 23·0 % of the radioactivity of the water-soluble rnetabolites was in glyoxylic acid. Radioactive glycine and glyoxylic acid, isolated from Ribes sativum 6 hr after uptake of 2,4-D-1-14C, contained essentially all of the 14C in the carboxyl-carbon atoms. When 2,4-D-2-14C was the precursor, the glycine isolated contained 64·8 % of its radioactivity in C2, while 60·0 % of the radioactivity in glyoxylic acid was in C2. The side-chain label of 2,4-D-2-14C-4-36Cl was more efficiently incorporated into ethanol-insoluble plant residue than the ring-label. The metabolism of glyoxylic acid-1-14C and 2,4-D-1-14C in excised Ribes sativum leaves were compared. The data suggest a cleavage of the acetate-moiety of 2,4-D resulting in a C2 compound, perhaps glyoxylate. |
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Keywords: | Grossulariaceae 2 4-dichlorophenoxyacetic acid metabolism glyoxylic acid glycine |
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