Expression of the Chlamydomonas reinhardtii Sedoheptulose‐1,7‐bisphosphatase in Dunaliella bardawil leads to enhanced photosynthesis and increased glycerol production |
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Authors: | Lei Fang Hui Xin Lin Chin Seng Low Mei Hui Wu Yvonne Chow Yuan Kun Lee |
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Institution: | 1. Department of Microbiology, National University of Singapore, , Singapore, Singapore;2. Institute of Chemical and Engineering Sciences, Agency for Science, Technology and Research, , Jurong Island, Singapore |
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Abstract: | Bioengineering of photoautotrophic microalgae into CO2 scrubbers and producers of value‐added metabolites is an appealing approach in low‐carbon economy. A strategy for microalgal bioengineering is to enhance the photosynthetic carbon assimilation through genetically modifying the photosynthetic pathways. The halotolerant microalgae Dunaliella posses an unique osmoregulatory mechanism, which accumulates intracellular glycerol in response to extracellular hyperosmotic stresses. In our study, the Calvin cycle enzyme sedoheptulose 1,7‐bisphosphatase from Chlamydomonas reinhardtii (CrSBPase) was transformed into Dunaliella bardawil, and the transformant CrSBP showed improved photosynthetic performance along with increased total organic carbon content and the osmoticum glycerol production. The results demonstrate that the potential of photosynthetic microalgae as CO2 removers could be enhanced through modifying the photosynthetic carbon reduction cycle, with glycerol as the carbon sink. |
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Keywords: | Microalgae
Dunaliella bardawil
photosynthesis sedoheptulose 1 7‐bisphosphatase
CO
2
glycerol |
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