不同油茶无性系炭疽病抗性及主要生理指标分析

为选育对炭疽病高抗的油茶(Camellia oleifera)良种，以5个闽西当地的油茶无性系为材料，采用病情指数法进行调查，评价其炭疽病抗性，并测定各无性系叶片的过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及可溶性蛋白含量等指标，分析油茶炭疽病抗性与其主要生理指标的相关性。结果表明，参试的5个油茶无性系中，高抗无性系1个(新罗2号)、中抗无性系3个(河田2号、红山1号、漳平2号)、低感无性系1个(武平2号)；不同无性系之间的POD、SOD、CAT活性及可溶性蛋白含量差异均达极显著水平(P<0.01)。相关性分析显示，POD活性、SOD活性、可溶性蛋白含量与油茶炭疽病的感病指数成负相关，CAT活性与感病指数的相关性未达显著水平。综上所述，不同油茶无性系对炭疽病的抗性各不相同，且其主要生理指标与抗性有一定的相关性，其中新罗2号无性系对炭疽病抗性强，主要生理指标也相对较高。该结果为油茶抗炭疽病良种选育及抗病机理研究提供依据。

Evaluation of Resistance of Different Camellia oleifera Clones to Anthracnose and Main Physiological Indexes inLeaves

In order to select better Camellia oleifera varieties with strong resistance to anthracnose, five C. oleifera Clones in western Fujian province were investigated by disease index method to evaluate their resistance to anthracnose, and the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and soluble protein content in the leaves of each clone were measured and compared. On this basis, the internal relationship between anthracnose resistance of C. oleifera and its main physiological indexes was explored through correlation analysis. The results showed that there were one high resistance clone (Xinluo No. 2), there medium resistance clones (Hetian No. 2, Hongshan No. 1, Zhangping No. 2) and one low sensitivity clone (Wuping No. 2) among the five C. oleifera clones tested for anthracnose resistance. And the differences of POD activity, SOD activity, CAT activity and soluble protein content among different clones reached a very significant level (P<0.01). Furthermore, correlation analysis showed that POD activity, SOD activity and soluble protein content were negatively correlated with the susceptibility index of Camellia anthracnose, while CAT activity was not a significant correlated with the susceptibility index slightly. In conclusion, C. oleifera clones had different resistance to anthracnose, and their main physiological indexes had a certain correlation with anthracnose resistance. Among these five C. oleifera clones, Xinluo No. 2 clone had strongest resistance to anthracnose, and its main physiological indexes were relatively higher too. These results would provide a basis for the breeding of improved varieties of C. oleifera resistant to anthracnose and the study of disease resistance mechanism.