金线莲HPLC指纹图谱研究

以14批不同来源金线莲药材为原料，采用高效液相色谱法(HPLC)，乙腈-甲酸水溶液为流动相，梯度洗脱，建立栽培及野生金线莲(Anoectochilus roxburgii)的指纹图谱，结合相似度分析，探讨金线莲与银线莲(Goodyera hachijoensis)的差异。结果表明，金线莲HPLC指纹图谱标定了24个共有峰，并指认了金线莲苷和葫芦巴碱、鼠李素-3-O-芸香糖苷、槲皮素、山柰酚、异鼠李素、Batatasin-Ⅲ等6个共有峰。除3批药材外，其余11批相似度均高于0.910，相似度总体较好。银线莲图谱相似度仅达0.529、0.721、0.698，均低于14批金线莲样品间的相似度。根据相似度评价，认为金线莲与银线莲存在差异，该结果可为分析评价金线莲与银线莲差异提供参考。

HPLC Fingerprinting of Anoectochilus roxburgii

In this experiment, the fingerprints of common cultivated and wild Anoectochilus roxburgii including 14 batches of herbs from different sources were established by high performance liquid chromatography (HPLC) with gradient elution using acetonitrile-formic acid aqueous solution as the mobile phase. The results showed that the HPLC fingerprint of A. roxburgii confirmed 24 common peaks including 6 common peaks of kinsenoside and trigonelline, rhamnetin-3-O-rutinoside, quercetin, kaempferol, isorhamnetin and batatasin-III. Except for 3 batches of medicinal materials, the similarity of the other 11 batches was higher than 0.910, and the similarity was generally good. The similarity of Goodyera hachijoensis only reached 0.529, 0.721, and 0.698, all of which were lower than the similarity within 14 batches of A. roxburgi. Based on the similarity evaluation, it was concluded that differences existed between A. roxburgii and G. hachijoensis. The results provide a reference for the analysis and evaluation of the differences between A. roxburgii and G. hachijoensis.