Purification of erythrocyte cytochrome P-450 from goat and chick. |
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| Authors: | S S Patil S J Makhija S S Pawar |
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| Affiliation: | Chemistry Department, S.B.E.S. Science College, Aurangabad. |
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| Abstract: | Cytochrome P-450 has been purified from goat and chick erythrocytes and characterized. Goat erythrocyte cytochrome P-450 content was higher than that of chick erythrocytes cytochrome P-450. Elution profile of purified protein from DEAE-cellulose column showed a single peak. The catalytic activities of aminopyrine-N-demethylase and acetanilide hydroxylase were found to be higher in purified proteins. Molecular weight was determined by SDS-polyacrylamide gel electrophoresis. |
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