PfoI, a unique type II restriction endonuclease that recognises the sequence 5′-T↓CCNGGA-3′ |
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| Authors: | M. Gaigalas, Z. Manelien&#x , R. Kazlauskien&#x , M. Petru&#x yt&#x , A. Janulaitis |
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| Affiliation: | M. Gaigalas, Z. Manelienė, R. Kazlauskienė, M. Petrušytė, and A. Janulaitis |
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| Abstract: | A new type II restriction endonuclease designated PfoI has been partially purified from Pseudomonas fluorescens biovar 126. PfoI recognises the interrupted hexanucleotide palindromic sequence 5′-T↓CCNGGA-3′ and cleaves DNA to produce protruding pentanucleotide 5′-ends. |
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