PfoI, a unique type II restriction endonuclease that recognises the sequence 5′-T↓CCNGGA-3′ |
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| Authors: | M Gaigalas Z Manelien&#x; R Kazlauskien&#x; M Petru&#x;yt&#x; and A Janulaitis |
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| Institution: | M. Gaigalas, Z. Manelien?, R. Kazlauskien?, M. Petru?yt?, and A. Janulaitis |
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| Abstract: | A new type II restriction endonuclease designated PfoI has been partially purified from Pseudomonas fluorescens biovar 126. PfoI recognises the interrupted hexanucleotide palindromic sequence 5′-T↓CCNGGA-3′ and cleaves DNA to produce protruding pentanucleotide 5′-ends. |
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