Cell fusion corrects the 4-nitroquinoline 1-oxide sensitivity of Werner syndrome fibroblast cell lines |
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| Authors: | PR Prince CE Ogburn MJ Moser MJ Emond GM Martin RJ Monnat Jr |
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| Institution: | (1) University of Washington, Department of Pathology, Box 357705, Seattle, WA 98195–7705, USA,;(2) University of Washington, Department of Genetics, Seattle, WA 98195, USA,;(3) University of Washington, Department of Biostatistics, Seattle, WA 98195, USA, |
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| Abstract: | We have shown that Werner syndrome (WRN) fibroblast cell lines are unusually sensitive to the DNA-damaging agent 4-nitroquinoline
1-oxide (4NQO), though not to gamma radiation or to hydrogen peroxide. The fusion of 4NQO-sensitive WRN and 4NQO-resistant
control fibroblast cell lines generated proliferating WRN × control cell hybrids that expressed WRN protein and were 4NQO-resistant.
These results establish the recessive nature of 4NQO sensitivity in WRN cell lines and provide a cellular assay for WRN protein
function.
Electronic Publication |
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