Effect of ghrelin on human endothelial cells apoptosis induced by high glucose |
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Authors: | Zhao Hong Liu GuoLiang Wang QiuYue Ding LiYing Cai Hui Jiang HaiHong Xin ZhongQiu |
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Institution: | Department of Endocrinology, First Hospital of China Medical University, ShenYang 110001, China. |
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Abstract: | Endothelial dysfunction is thought to be a major cause of vascular complications in diabetes. Our research shows that ghrelin attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells (ECV-304). Exposure to glucose (33.3mM) for 72 h caused a significant increase in apoptosis, as evaluated by TUNEL and flow cytometry, but pretreatment of ghrelin (10(-7)M) eliminated high glucose-induced apoptosis in ECV-304. Ghrelin also prevented the induction of caspase-3 activation, in cells incubated with glucose (33.3 mM). Exposure of cells to ghrelin (10(-7)M) caused rapid activation of Akt. PI3K inhibitor, LY294002 attenuated ghrelin's inhibitory effect on caspase-3 activity. Ghrelin protected endothelial cells from high glucose by inhibiting reactive oxygen species (ROS) generation. Results of our study indicate that ghrelin inhibits both high glucose-induced apoptosis via PI3K/Akt pathway and ROS production in ECV-304. This peptide may have potential in preventing diabetic complications, especially in obese patients. |
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Keywords: | Ghrelin Apoptosis Endothelial dysfunction Diabetes |
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