Identification of carotenoids with high antioxidant capacity produced by extremophile microorganisms |
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Authors: | Fernanda Mandelli Viviane S Miranda Eliseu Rodrigues Adriana Z Mercadante |
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Institution: | (1) Department of Food Science, Faculty of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, 13083-862, Brazil; |
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Abstract: | In this study, the carotenoids produced by the extremophile microorganisms Halococcus morrhuae, Halobacterium salinarium and Thermus filiformis were separated and identified by high-performance liquid chromatography connected to a diode array detector and a tandem
mass spectrometer. The in vitro scavenging capacity of the carotenoid extracts against radical and non-radical species was
evaluated. In halophilic microorganisms, the following carotenoids were identified: bacterioruberin, bisanhydrobacterioruberin,
trisanhydrobacterioruberin and their derivatives. In the thermophilic bacterium, the carotenoids all-trans-zeaxanthin, zeaxanthin monoglucoside, thermozeaxanthins and thermobiszeaxanthins were identified. The antioxidant capacities
of the carotenoid extracts of H. morrhuae (trolox equivalent antioxidant capacity = 5.07 and IC50 = 0.85 μg mL−1) and H. salinarium (trolox equivalent antioxidant capacity = 5.28 and IC50 = 0.84 μg mL−1) were similar and higher than those of the bacterium T. filiformis (trolox equivalent antioxidant capacity = 2.87 and IC50 = 2.41 μg mL−1). This difference is related to the presence of acyclic carotenoids with both large numbers of conjugated double bounds and
of hydroxyl groups in the major carotenoid of the halophilic microorganisms. |
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