Irradiated stem cells and ageing of the haematopoietic system |
| |
Authors: | Email author" target="_blank">Ji?ina?VávrováEmail author Zuzana??inkorová Martina??ezá?ová Ale??Tichy Stanislav?Filip Jaroslav?Mokry Emilie?Luká?ová |
| |
Institution: | 1.Department of Radiobiology, Faculty of Health Sciences,University of Defence in Brno,Hradec Králové,Czech Republic;2.Institute of Medical Biochemistry, Faculty of Medicine,Charles University in Prague,Hradec Králové,Czech Republic;3.Department of Oncology and Radiotherapy, Faculty of Medicine and Teaching Hospital,Charles University in Prague,Hradec Králové,Czech Republic;4.Department of Histology and Embryology, Faculty of Medicine,Charles University in Prague,Hradec Králové,Czech Republic;5.Institute of Biophysics,Czech Academy of Sciences,Brno,Czech Republic |
| |
Abstract: | In the work presented here, changes in haematopoiesis of mice (B6129SF2/J) were studied 1 year after their whole-body exposure
to a dose of 7 Gy (72% of mice survived). The irradiated mice were compared with non-irradiated younger (4 months of age)
and older (16 months of age) mice. There was a significant increase in the relative abundance of primitive stem cells with
long-term capability of the haematopoiesis recovery lin−/Sca-1+/CD117+/CD34− in the bone marrow of mice aged 16 months (irradiated and non-irradiated) compared with those aged 4 months. In terms of
the ability to respond to further whole-body irradiation at a dose of 1 Gy, the presence of γH2A.X foci was studied in lin− bone marrow cells. There was a considerable number of persisting foci in lin− stem cells isolated from the bone marrow of the older irradiated mice. In the blood count from the peripheral blood of the
older mice (both non-irradiated and irradiated at 7 Gy), there was a significant increase in granulocytes. In the group exposed
to 7 Gy, the numbers of thrombocytes significantly increased, and on the contrary, the numbers of erythrocytes, the amount
of haemoglobin, and haematocrit significantly decreased. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|